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Titre: | Development and Characterization of Microsatellite Loci for the Moroccan Endemic Endangered Species Argania spinosa (Sapotaceae) |
Autre(s) titre(s): | Développement et Caractérisation de Lieux Microsatellites pour les Espèces Endémiques Marocaines en voie de disparition Argania spinosa (Sapotaceae) |
Auteur(s): | Gaboun,F., INRA, Rabat (Ma) centre Régional de la Recherche Agronomique de Rabat Dauchot,N., Université de Namur, Bruxelles (Be) Cutsem,P.V., Université de Namur, Bruxelles (Be) El Bahloul,Y., INRA, Rabat (Ma) centre Régional de la Recherche Agronomique de Rabat Machtoun,I., INRA, Rabat (Ma) centre Régional de la Recherche Agronomique de Rabat |
Issue Date: | 2014 |
Référence bibliographique: | Applications in Plant Sciences, V.2(4) |
Résumé: | Argania spinosa (L.) Skeels is endemic to Morocco and is known worldwide for its oil, which is extremely rich in unsaturated fatty acids and used in the food and cosmetic industries. Argan is the only Sapotaceae species in the region. Argan forest is found primarily in the southwestern part of Morocco and in the Souss-Massa-Drâa region ( Msanda et al., 2005 ); a few populations have also been identifi ed in southwestern Algeria ( Kaabèche et al., 2010 ). Currently, argan is facing critical regeneration problems resulting from overgrazing of young sprouts and trees by goats, coupled with inadequate protection; consequently, argan tree genetic diversity is severely threatened. Molecular analyses of A. spinosa are very recent and still quite limited,mainly due to the lack of species-specifi c molecular tools. A few studies have tried to evaluate its genetic diversity, fi rst with the analysis of isozymes ( El Mousadik and Petit, 1996a ) and chloroplast DNA ( El Mousadik and Petit, 1996b ) and later with interspecific random-amplifi ed polymorphic DNA (RAPDs) and simple sequence repeats (SSRs) initially developed on two different Sapotaceae species ( Majourhat et al., 2008 ). However, only six SSRs were polymorphic, and these exhibited low diversity according to what would be expected in a perennial spontaneous (i.e., natural , nondomesticated) tree. Species-specific codominant molecular markers are thus critically needed. Here, we developed de novo, highly polymorphic and A. spinosa –specific SSR markers and used them to evaluate the genetic diversity of a collection of geographically diversified argan trees. |
License: | http://andzoa.ma/fr |
URI: | http://localhost:8080/dspace/handle/0/1473 |
Related document: | http://www.bioone.org/ |
ISSN: | 2168 - 0450 |
Appears in Collections: | Sans document
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