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Título : Structure génétique et collection modiale du germoplasme d'olivier à Marrakech: vers une gestion optimisée et l'utilisation des ressources génétiques d'olive méditerranéenne
Autor : Khadari, B., INRA, UMR 1334 Amélioration Génétique et Adaptation des Plantes (AGAP), 34398 Montpellier
Cherkaoui, E.
Oukabli, A., INRA, Meknes (Ma) centre Régional de la Recherche Agronomique de Meknes
Haouane , H.
El Bakkali, A., INRA,Tanger (Ma) centre Régional de la Recherche Agronomique de Tanger
Tollon Sylvain Santoni, C.
Moukhli, A., INRA, Marrakech (Ma) centre Régional de la Recherche Agronomique de Marrakech
Fecha de publicación : 2011
Citación : Genetica, No Elecronique
Resumen : The conservation of cultivated plants in ex-situ collections is essential for the optimal management and use of their genetic resources. For the olive tree, two world germplasm banks (OWGB) are presently established, in Cordoba (Spain) and Marrakech (Morocco). This latter was recently founded and includes 561 accessions from 14 Mediterranean countries. Using 12 nuclear microsatellites (SSRs) and three chloroplast DNA markers, this collection was characterised to examine the structure of the genetic diversity and propose a set of olive accessions encom¬passing the whole Mediterranean allelic diversity range. We identi& 64257;ed 505 SSR pro& 64257;les based on a total of 210 alleles. Based on these markers, the genetic diversity was similar to that of cultivars and wild olives which were previously characterised in another study indicating that OWGB Marrakech is representative of Mediterranean olive germplasm. Using a model-based Bayesian clustering method and principal components analysis, this OWGB was structured into three main gene pools corresponding to eastern, central and western parts of the Mediterranean Basin. We proposed 10 cores of 67 accessions capturing all detected alleles and 10 cores of 58 accessions capturing the 186 alleles observed more than once. In each of the 10 cores, a set of 40 accessions was identical, whereas the remaining accessions were different, indicating the need to include complementary criteria such as phenotypic adap¬tive and agronomic traits. Our study generated a molecular database for the entire OWGB Marrakech that may be used to optimise a strategy for the management of olive genetic.
License: http://andzoa.ma/fr
URI : http://webagris.inra.org.ma/doc/elbakkali09111.pdf
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