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Título : Prototypic and molecular characterization of date Palm off-types
Autor : Tripler, E, Southern Arava Research Station, Hevel Eilot (Israel)
Cohen, Y., Agricultural Research Organization, Bet Dagan (Israel)
Korchinsky, R., Agricultural Research Organization, Bet Dagan (Israel)
Gurevich, V., Agricultural Research Organization, Bet Dagan (Israel)
Lavi, U, Agricultural Research Organization, Bet Dagan (Israel)
Fecha de publicación : 2005
Resumen : Propagation of date palms using tissue culture techniques is very advantageous. However, among the normal trees, many abnorrnal, off-type trees are generated. The present study applies Amplified Fragment Length Polymorphism (AFLP) to characterize three common date paim off-type phenotypes widely spread among tissue culture originated trees in Israel, and in other countries. These phenotypes include variegation, "low level fruit setting" and dwarfism. While some off-types are easily detected in young plantlets, these off-types are commonly detected in the field years after planting. The last two, tend to occur in mass numbers mainly in plantlets of specific tissue culture laboratories. Therefore, both preventing the generation and developing methods for early detection of these off-types are necessary. Wà®thin the variegated trees multiple mutations seems to occur. In contrast, although relatively low number of mutations was detected in the fruit setting and the dwarf trees, no specific mutation was found b be associated with these phenotypes. However, differences in DNA-methylation patterns seem to characterize these off-types. Reduction in the overail DNA methylation level seerns to be associated with both the "low fruit set" and the dwarf phertotypes.
License: http://andzoa.ma/fr
URI : http://webagris.inra.org.ma/doc/ouvrages/truetype....
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